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Immobilization of collagen peptide on dialdehyde bacterial ...

Jul 21, 2015· From Figure 6B, it is observed that the peptide-binding rate increased with the enhancement of pH value. For instance, DBC with DO of 59.7% resulted in a peptide-binding rate of 31.26% at pH =6 as compared with 46.79% at pH =7 and 54.99% at pH =8. Higher DO of DBC could facilitate a higher peptide-binding rate.

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Post-Assembly Functionalization of Supramolecular ...

Post-assembly functionalization of supramolecular nanostructures has the potential to expand the range of their applications. We report here the use of the chemoselective native chemical ligation (NCL) reaction to functionalize self-assembled peptide amphiphile (PA) nanofibers. This strategy can be used to incorporate specific bioactivity on the nanofibers, and as a model, we demonstrate ...

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Chemoselective Immobilization of Peptides on Abiotic and ...

Jul 19, 2012· jugation of linker 3 to CK(Biotin)-GGIKVAV 4 gave the target CyO-IVAV peptide in 71% yield (Scheme 2). To generate azide groups on the islet surface,6 islets were incubated in PP-N 3 (∼40% grafted, 80 μM, 40 min), rinsed to remove nonadsorbed copolymer, and incubated in CyO-IKVAV (100 μM, 1 h). Nonbound peptide was washed away, and islets

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Peptides - SlideShare

Jan 29, 2014· 3. -Peptides (from the Greek word means "digested") are short polymers of amino acid (monomers) linked by peptide bonds, the covalent chemical bonds formed between two molecules when the carboxyl group of one molecule reacts with the amino group of the other molecule.

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Analytical Mthoe ds

An elaborately designed peptide probe Ac-CCPGCAAAARRRR-NH 2 is employed here for assay of Zn 2+. This tridecapeptide consists of binding part (CCPGC), spacer (AAAA) and immobilization part (RRRR) to interact with CC SAM orientedly. We compared the sensitivity and the specificity of the linker molecules with that of common attachment agent

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Immobilization of sericin molecules via amorphous carbon ...

In this study, we focused on sericin hydrolysates, originating from silkworm used in serum-free human bone marrow-derived mesenchymal stem cells (hBM-MSCs) culture. We reported the effect of a covalent linkage between a bioactive protein molecule and polystyrene dish surface via a carbon intermediate layer which can slow down the release rate of protein compounds into the phosphate buffer ...

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use of bioactive peptides to modify materials for bone ...

The immobilization of certain molecules on scaffolds has been shown very effective to improve not only recruiting stem cells but also triggering their osteogenic differentiation because these molecules can provide osteogenesis-stimulating signals for the cells [4, 11]. The practical advantage of using peptides, rather than native proteins, for ...

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User-defined, temporal presentation of bioactive molecules ...

The immobilization of biomolecules onto or within tissue culture substrates is important when engineering environments that mimic the ECM. 18–21 One successful approach for incorporating bioactive molecules into scaffolds is photo-mediated thiol–ene click conjugation. 22–26 Modifying hydrogel-forming polymers with norbornene groups ...

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Immobilization of Enzymes on Magnetic Particles - Enzyme ...

Dec 14, 2020· 1. Dissolve the protein that is to be coupled in phosphate buffer pH 6.3 at a concentration of 20 mg/mL. Fig. 4. Scheme for preparation of fine magnetic particles. 2. Separately weigh and dissolve CDI in phosphate buffer, pH 6.3, at a concentration of 20 mg/mL. 3. Mix 1 mL of protein (20 mg/mL) with 1 mL of CDI (20 mg/mL) dissolved in buffer ...

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Single-Step Conjugation of Bioactive Peptides to Proteins ...

Oct 21, 2009· Thus, to determine whether the OSu-activated ester of the bis-arylhydrozone moiety of one EP67 peptide can react with the free ε-amino group on the Lys side-chain of another EP67, 2.1 mg of 7 was dissolved in 1 ml of 0.1 M phosphate buffer pH …

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Surface immobilization and bioactivity of TGF-β1 inhibitor ...

organic linking molecules such as silanes 21,22, phosphonates 23,24, thiols 24 and polyethylene glycol (PEG) 25 have been studied to decorate surfaces with biological molecules. 3-aminopropyltriethoxy silane (APTES) has been widely used to tether bioactive peptides to a number of oxide surfaces 26,27. However, the use of this silane for this ...

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Rapid screening and identification of bioactive compounds ...

Sep 24, 2018· San-ao decoction (SAD) has been widely used in Chinese medicine against respiratory diseases, such as asthma and rhinallergosis. The bioactive compounds for such pharmacological action remain unknown. We developed a methodology to isolate the bioactive compounds of SAD. The assay involved the immobilization of beta 2-adrenoceptor (β2-AR) onto magnetic fine particles, the …

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Immobilization of acetylcholinesterase in nanofibrous PVA ...

Feb 17, 2010· The fibers were washed three times thoroughly with phosphate buffer solution (25 mM, pH 7.4) to extract out un‐bonded enzymes. Buffer solutions were found to have no detectable enzyme activity, indicating that there was negligible enzyme leakage during washing procedure. Therefore, the yield of AChE immobilization was found to be about .

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Journal of Yasuda et al, J Biosens Bioelectron 201, :4 ...

of the protein solutions was regulated using either 10 mM of sodium acetate (pH 3.8), sodium phosphate (pH 7.0), or sodium borate (pH 9.0) as buffer solutions. In the adsorption step, 10 µM of protein stock solution in water, polymer particle suspended in buffer solution, 100 mM buffer stock solution, and Milli-Q water until the final buffer

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A photoreversible protein-patterning approach for guiding ...

Feb 23, 2015· where is the quantum yield (determined to be 0.73 for N 3 –TEG–ONH–NPPOC, Supplementary Fig. 2), ɛ is the molar absorptivity of the sample (measured at …

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Immobilization of collagen peptide on dialdehyde bacterial ...

Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration Xiaoxiao Wen,1 Yudong Zheng,1 Jian Wu,2 Lu-Ning Wang,1 Zhenya Yuan,1 Jiang Peng,3 Haoye Meng3 1School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, People's Republic of China; 2Suzhou Institute of Nano ...

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Functionalization of hydrophobic surfaces with ...

Layer-by-layer deposition is a common physical immobilization technique applied to hydrophilic materials such as metal oxides, mainly due to electrostatic interactions 16,17 or the adding of a specific surface binding recognition sequence to the peptide. 18–20 This method may be applied on hydrophobic polymer surfaces after applying a process ...

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Functionalization of Cellulose-Based Hydrogels with Bi ...

Jun 09, 2021· All protein dilutions were prepared in Tris-Saline Tween (TST) buffer (50 mM Tris buffer pH 7.6, 150 mM NaCl, 0.05% v/v Tween 20), unless stated otherwise. Phosphate-buffered saline (PBS; 10 mM phosphate pH 7.2, 150 mM NaCl) was used in the to dilute the oligonucleotide.

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(PDF) Electrochemical Polymerization of (2-Dodecyl-4, 7-di ...

Electrochemical Polymerization of (2-Dodecyl-4, 7-di (thiophen-2-yl)-2H-benzo[d][1,2,3] triazole): A Novel Matrix for Biomolecule Immobilization Macromolecular Bioscience, 2010 Levent Toppare

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Covalent Immobilization of Affinity Ligands | Thermo ...

NHS esters are reactive groups formed by EDC activation of carboxylate molecules. NHS ester-activated resins react with primary amines in slightly alkaline conditions (pH 7.2-8.5) to yield stable amide bonds. The immobilization reaction is usually performed in phosphate buffer at pH 7.2-8.0 for 0.5 to 4 hours at room temperature or 4°C.

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Immobilization of a bone and cartilage stimulating peptide ...

Nov 22, 2007· A synthetic peptide fragment of human collagen type I (BCSP™-1) was linked to the surface of a commercially available ceramic in an effort to improve the properties of the bone graft substitute to accelerate local healing. BCSP™-1 was covalently immobilized on the surface of the ceramic via the linkers 3-aminopropyl-triethoxysilane (APTES) and suberic acid bis-N …

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Chemoselective immobilization of peptides on abiotic and ...

Jun 01, 2010· Strain promoted azide-alkyne cyclooaddition was performed by incubating the slide with CyO-IKVAV at 50 and 25 μM concentrations in phosphate buffer for 15 min. Detection of immobilized peptide was accomplished by incubating the slide with Alexa488-streptavidin (5 μg/mL) for 1 h followed by fluorescence detection at 495 nm/519 nm (Ex/Em) with ...

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An XPS study on the covalent immobilization of adhesion ...

Nov 01, 2010· The silanized samples were immersed in 1% glutaraldehyde solution in 0.1 M phosphate buffer for 4 h at 25 °C, then washed with buffer and dried under vacuum. Finally, the glasses were immersed overnight into a peptide solution (concentration 1 mg/ml) in phosphate buffer. Pathway B: selective immobilization (sample 4: HVPs; sample 6: RGDs)

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CarboxyLink™ Coupling Gel (Immobilized …

for immobilization of peptides for use in affinity purification of antibodies or other binding partners. Although other amine-reactive methods can be used for attachment of molecules to Immobilized DADPA, the carbodiimide EDC is unique among cross-linkers in enabling conjugation through carboxylates. In near-neutral to acidic pH buffer, EDC

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Plasma treatment in air at atmospheric pressure that ...

Jul 15, 2020· Applications of this linker-free immobilization approach for protein microarrays,, and bioactive medical implants,,,, have been demonstrated. The longevity of the surface's covalent coupling capability is proportional to the depth of the modified radical containing surface layer . Appropriate tuning of plasma parameters to achieve ...

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Chemoselective Immobilization of Peptides on Abiotic and ...

Jun 01, 2010· Strain promoted azide-alkyne cyclooaddition was performed by incubating the slide with CyO-IKVAV at 50 and 25 μM concentrations in phosphate buffer for 15 min. Detection of immobilized peptide was accomplished by incubating the slide with Alexa488-streptavidin (5 μg/mL) for 1 h followed by fluorescence detection at 495 nm/519 nm (Ex/Em) with ...

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Alex C. Chen

immobilization onto a surface. This hypothesis is supported through in vitro analysis of cytokines released in macrophage culture media and RT-PCR analysis of genes correlating with M2 polarization. An in vivo pilot study of subcutaneous implants with immobilized bioactive molecules also …

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Immobilization of Neurotrophin Peptides on Gold ...

Aug 01, 2017· To remove unloaded peptide molecules from the peptide/SUV systems, Amicon Ultra-0.5 ultracentrifuge filters (Sigma-Aldrich) were used to centrifuge (14 000 rpm for 30 min at 25 °C) and the recovered solutions were characterized . The SUV and peptide/SUVs were stored under Ar and used within 2 weeks, according to an established protocol.

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Protein Immobilization - an overview | ScienceDirect Topics

A comparatively stable method of protein immobilization is to link a protein to the substrate via a covalent bond. Functional groups, i.e., carboxylic -COOH, hydroxyl -OH, amine -NH 2 and thiol -SH groups, are usually involved to covalently bind biomolecules on a solid support. The functional groups present on the surface of the material usually form bonds with groups present in the exterior ...

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Amine-Reactive Crosslinker Chemistry | Thermo Fisher ...

Hydrolysis of the NHS ester competes with the primary amine reaction. The rate of hydrolysis increases with buffer pH and contributes to less-efficient crosslinking in less-concentrated protein solutions. The half-life of hydrolysis for NHS-ester compounds is 4 to 5 h at pH 7.0 and 0°C. This half-life decreases to 10 mins at pH 8.6 and 4°C.